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B2025
Sigma
Brilliant Blue G - Colloidal Concentrate
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Analysis Note | This product has been tested for suitability on SDS-PAGE. |
| Application | Brilliant Blue G – colloidal stains protein in polyacrylamide and agarose gels (IEF) and provides approximately a 10-fold increase in sensitivity (with fixation) over methanol/acetic acid based Brilliant Blue G and R. Fixing (F7264) is required prior to staining. |
| Reconstitution | The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water. |
Safety
References
| reference | Neuhoff, et al., Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9, 255-262, (1988) Abstract |
